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目的探讨隔日限食对高脂诱导的肥胖小鼠胰岛β细胞功能的影响。方法 30只C57BL/6小鼠随机均分为标准饮食组(STD组)、高脂饮食组(HFD组)和高脂饮食+限食组(HFDCR组,采取隔日限食法)。限食干预4个月后,检测小鼠FBG、TC、TG、糖耐量及血清胰岛素水平;HE染色观察胰腺组织胰岛形态和结构;Hoechst染色检测胰岛细胞凋亡;免疫荧光检测自噬标记蛋白微管相关蛋白轻链3(LC3)的表达。结果HFD组小鼠体重、FBG、TC、糖耐量曲线下面积均高于STD组和HFDCR组(P<0.05)。HFDCR组血清胰岛素水平高于STD组(P<0.05)。STD组和HFDCR组小鼠胰岛细胞密度高于HFD组,且胰岛形态和结构完整性也优于HFD组。HFDCR组胰岛细胞细胞凋亡率低于HFD组(P<0.05),而自噬标记蛋白LC3的表达水平高于HFD组(P<0.05)。结论限食治疗能够保护高脂诱导的肥胖小鼠胰岛β细胞功能,该作用可能与限食诱导的β细胞自噬水平上调相关。
Objective To investigate the effect of daily restriction on the function of islet β cells in obese mice induced by high fat diet. Methods Thirty C57BL / 6 mice were randomly divided into standard diet group (STD group), high fat diet group (HFD group) and high fat diet group (HFDCR group). After four months of food restriction, the levels of FBG, TC, TG, glucose tolerance and serum insulin in mice were measured. The morphology and structure of pancreatic islets were observed by HE staining. The apoptosis of pancreatic islets was detected by Hoechst staining. Tube-associated protein light chain 3 (LC3) expression. Results The body weight, FBG, TC, and area under the glucose tolerance curve of HFD group were significantly higher than those of STD group and HFDCR group (P <0.05). The level of serum insulin in HFDCR group was higher than that in STD group (P <0.05). The density of islet cells in STD group and HFDCR group was higher than that in HFD group, and the morphological and structural integrity of islets was also better than HFD group. The apoptosis rate of islet cells in HFDCR group was lower than that in HFD group (P <0.05), while that of autophagy marker protein LC3 was higher than that in HFD group (P <0.05). Conclusion Food restriction treatment can protect the islet β-cell function of obese mice induced by high fat diet, which may be related to the up-regulation of autophagy induced by diet-restricted β-cell.