本文采用5种方法(传统CTAB法、改良CTAB法、SDS法、高盐低p H法和试剂盒法)提取砂生槐叶片DNA,利用分光光度法检测DNA的浓度与纯度,并进行RAPD-PCR扩增检测,旨在探讨砂生槐叶片DNA的最佳提取方法。结果表明改良CTAB法速度较快,便于操作,提取的DNA质量好,RAPD-PCR扩增条带清晰,适用于砂生槐叶片DNA的提取方法。 In this paper, five kinds of methods (traditional CTAB method, modified CTAB method, SDS method, high salt and low p H method and kit method) were used to extract the DNA from the leaves of Sophora saponaria. The concentration and purity of DNA were detected by spectrophotometry, and RAPD- PCR amplification detection, to explore the best method for extracting DNA from the leaves of Sophora saponins. The results showed that the modified CTAB method was rapid and easy to operate. The extracted DNA was of good quality and RAPD-PCR amplified bands were clear.