论文部分内容阅读
以金丝小枣的优良品系A27为试材,对其茎段组培快繁过程中有关技术进行了研究。结果表明,采接外植体以5月中旬的嫩枝枣头为宜,7天内可生长新芽。继代快繁过程中,附加0.3mg/1的IBA可促进生根,附加6-BA0.2mg/1+IBA0.5mg/1则有利于长芽展叶。1年中最佳繁殖时期为5月份,4月份次之。含顶芽茎段和含叶腋芽茎段在枣组培快繁时对试管苗生长无明显差异。
A27, an excellent line of golden jujube, was used as experimental material to study the technique of tissue culture and propagation of its stem segments. The results showed that the explants were suitable for shoots in mid-May and sprouts in 7 days. In the process of subculture, the addition of 0.3mg / l IBA could promote rooting, and the addition of 6-BA0.2mg / 1 + IBA0.5mg / The best breeding period in 1 year was May, followed by April. There were no significant differences in the growth of test tube seedlings between the buds containing the terminal buds and the axillary bud stem segments containing leaves when they were cultured rapidly.