论文部分内容阅读
本文比较分析了蔗糖、麦芽糖、乳糖、葡萄糖、果糖、半乳糖６种碳源和１７种基因型对棉花未受精胚珠纤维离体诱导和生长发育的影响。研究结果表明，０．２ｍｏｌ·Ｌ－１葡萄糖是诱导棉花未受精胚珠纤维离体发育合适的碳源。在亲本中珂字棉３１２和超鸡脚叶棉未受榆胚珠的离体纤维的诱导效果最好，诱导率分别为７５．４％和７９．２％。在杂种Ｆ１中，棕絮棉×绿絮棉Ｆ１，珂字棉×棕絮棉Ｆ１和棕絮棉×珂字棉Ｆ１的未受精胚珠的离休纤维诱导率和纤维生长量都较好。在沟棉３号×中６２１Ｆ１和泗棉３号×豫棉４２２Ｆ１中，前者的纤维诱导率在所有基因型中为最高，后者的纤维生长量则在所有的处理中为最大。未试验结果表明，离休诱导棉花未受精胚珠的纤维发育要有合适的培养基和基因型。
In this paper, the effects of sucrose, maltose, lactose, glucose, fructose, galactose and seventeen genotypes on in vitro induction and growth of cotton unfertilized ovule were compared. The results showed that 0.2mol·L-1 glucose was a suitable carbon source for in vitro development of cotton unfertilized ovule fibers. The best in vitro induction of the off-fiber from the Cynomolgus cotton 312 and the super-chicken foot-leaf cotton without the ovules was 75.4% and 79.2%, respectively. In hybrid F1, the retired fiber induction rate and fiber growth of the unfertilized ovules of brown cotton × cotton × F1, cotton × cotton × brown cotton × cotton × cotton × cotton × mianxian F1 were all better. In Gumian 3 × 621F1 and Simian 3 × Yumian 422F1, the former had the highest fiber induction rate in all genotypes, and the latter was the largest in all treatments. The untested results show that there is a suitable medium and genotype for the fiber development induced by retired cotton unfertilized ovules.