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目的探讨蒙古黄芪2种不同成分提取物对肠道微生态失调小鼠的调节作用。方法从60只昆明种清洁级小鼠中随机取出10只作为正常对照组,其余50只每天灌胃0.3 g/ml的盐酸林可霉素,每日2次,每次0.3 ml,连续3 d,诱导小鼠肠道微生态失调,正常对照组以等量生理盐水处理。第4天除正常对照组外,所有小鼠均灌胃造成肠道微生态失调模型,将模型小鼠随机分为4组:黄芪多糖组、黄芪皂苷组、丽珠肠乐组和自然恢复组,并灌胃相应浓度药物进行调整治疗,自然恢复组以等量生理盐水处理,连续7 d。结果与自然恢复组相比,黄芪多糖、黄芪皂苷2种药物均能扶植模型小鼠肠道正常菌群生长,提高乙酸含量,降低内毒素水平,有效控制肠杆菌向肝脏的易位(P<0.05);黄芪多糖的各项检测指标与其他组相比,差异有显著性,有统计学意义(P<0.05)。结论蒙古黄芪不同成分提取物对肠道微生态失调小鼠均具有一定的调节作用,黄芪多糖的疗效可能优于黄芪皂苷。
Objective To investigate the regulation effect of two different constituents of Astragalus membranaceus on intestinal microflora in mice. Methods Ten rabbits were randomly selected from 60 Kunming mice as normal control group. The other 50 rabbits were orally administered with 0.3 g / ml lincomycin twice a day for 0.3 days for 3 days , Induced intestinal microbiological imbalance in mice, the normal control group with the same amount of saline treatment. On the 4th day, all the mice except the normal control group were gavaged with gut microecology model. The model mice were randomly divided into 4 groups: Astragalus polysaccharide group, Astragaloside group, Ligustrum group and natural recovery group , And gavage the corresponding concentration of drugs for adjustment and treatment, natural recovery group treated with equal amount of saline for 7 days. Results Compared with the natural recovery group, both APS and Astragalus saponin could promote the growth of normal intestinal flora in model mice, increase the content of acetic acid, decrease the level of endotoxin and effectively control the translocation of Enterobacteriaceae to the liver (P < 0.05). The detection indexes of APS were significantly different from other groups (P <0.05). Conclusion Mongolian astragalus extract of different components of intestinal micro-ecological disorders in mice have a certain regulatory effect, the efficacy of astragalus polysaccharides may be superior to Astragaloside.