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用限制性内切酶ＰｓｔⅠ消化人型结核杆菌Ｈ３７ＲｖＤＮＡ，与质粒ｐＵＣ１９ＤＮＡ连接后转化大肠杆菌，经同位素３２Ｐ标记的人型结核杆菌Ｈ３７Ｒｖ全染色体ＤＮＡ探针筛选出６个阳性克隆（ＭＴ１～６），其中克隆ＤＮＡ片段ＭＴ２（４．２Ｋｂ）、ＭＴ４（３．５Ｋｂ）和ＭＴ５（３．０Ｋｂ）标记成探针，检测了２２种分支杆菌标准株和１５株人型结核杆菌临床分离株以及２种非分支杆菌，其杂交结果完全相同，与人型结核杆菌及其临床分离株杂交阳性，与牛型结核杆菌、卡介苗、堪萨斯分支杆菌、鸟分支杆菌、胃分支杆菌、龟分支杆菌和戈登分支杆菌ＤＮＡ有交叉杂交，与其它１２种分支杆菌以及大肠杆菌、星状诺卡氏菌不杂交。克隆ＤＮＡ探针可能成为分支杆菌分类、鉴定的一种新工具。
The H37Rv DNA of human Mycobacterium tuberculosis was digested with restriction endonuclease PstⅠ, then ligated with plasmid pUC19DNA and transformed into E.coli. Six positive clones (MT1 ~ 6) were screened by isotope 32P-labeled Mycobacterium tuberculosis H37Rv whole chromosome DNA probe, Clone DNA fragments MT2 (4.2Kb), MT4 (3.5Kb) and MT5 (3.0Kb) labeled as a probe, detected 22 kinds of standard strains of Mycobacterium tuberculosis and 15 strains of Mycobacterium tuberculosis clinical isolates and two Non-mycobacterium, the hybridization results are identical, and Mycobacterium tuberculosis and clinical isolates hybrid positive, and the bovine Mycobacterium tuberculosis, BCG, Mycobacterium Kansas, Mycobacterium, Mycobacterium, Mycobacterium turtle and Gordon branch Bacillus DNA cross-hybridization, and other 12 kinds of mycobacteria and Escherichia coli, Nocardia non-hybridization. Cloning of DNA probes may become a new tool for the identification and identification of mycobacteria.