论文部分内容阅读
本实验用Northern杂交方法,以c-myc癌基因为探针,对人和小鼠骨髓瘤细胞以及杂交后的同种和异种胞质体杂交细胞和异种杂交细胞进行了癌基因表达的核酸分子杂交分析。结果表明,在小鼠-小鼠,小鼠-兔及人-小鼠的胞质体杂交细胞(BW-RM,BW-RR,HMy-RM)中均未检测到myc癌基因的表达产物。但自15代以后开始出现微弱或可测的表达活性,并随传代而有上升的趋向。同样,在小鼠浆细胞瘤(Sp2/o)与大鼠有核红细胞(ER)的异种杂交中,杂交细胞(SpER)的第4代和第7代细胞中亦未检查到myc基因转录物。表明细胞杂交后,myc基因受到了抑制。这种抑制作用似无种属特异性。这一结果提示红系细胞内“胞质因子”对肿瘤细胞原来活化的myc基因具有抑制作用,并与杂交细胞的恶性下降有关。论文对胞质因子对癌基因表达的调控作用及其分子生物学机理进行了讨论。
In this experiment, oncogene expression of human and mouse myeloma cells and hybridized homologous and xenogeneic hybrid cells and xenogeneic hybrid cells using a Northern hybridization method with c-myc oncogene as probes Hybridization analysis. The results showed that no expression product of myc oncogene was detected in mouse-mouse, mouse-rabbit and human-mouse cytoplasmic hybrid cells (BW-RM, BW-RR, HMy-RM). However, weak or measurable expression activity began to appear after 15 generations, and it increased with passage. Similarly, myc gene transcripts were not detected in the 4th and 7th generation cells of hybridoma cells (SpER) in heterozygous mouse plasmacytoma (Sp2/o) and rat nucleated red blood cells (ER). . It showed that myc gene was inhibited after cell hybridization. This inhibition appears to be species-specific. This result suggests that the “cytoplasmic factor” in erythroid cells has an inhibitory effect on the myc gene activated by the tumor cells and is related to the decline of the malignant cells. This paper discusses the regulation of cytosolic factors on the expression of oncogenes and the molecular biological mechanism.