论文部分内容阅读
目的了解曾发生登革热流行的旧疫区健康人群抗登革病毒的抗体水平及分布。方法在1986年曾爆发Ⅱ型登革热疫情的黄埔区鱼珠街一带,对2周内无临床症状的健康人群横断面采样,收集血清,ELISA法检测血清中抗登革病毒IgG抗体,并和非疫点健康人群抗登革病毒IgG抗体阳性率进行比较。RT-PCR法检测抗体阳性者血清中登革病毒。结果登革热疫点健康人群抗登革病毒IgG抗体阳性率为23.3%,非疫点健康人群抗登革病毒IgG抗体阳性率为3.8%,两者差异有统计学意义(P<0.01);登革热疫点人群抗体阳性率与年龄成正相关,0～20、21～30、31～40、41～50、51～60、61～70和70岁以上各年龄组的抗体阳性率分别为3.8%、5.8%、9.1%、26.5%、27.7%、32.5%和35.1%。30岁以下年龄组与31～40岁年龄组、31～40岁年龄组与41～60岁年龄组、41～60岁年龄组与61岁年龄组间的抗登革病毒IgG抗体阳性率差异分别具有统计学意义(P<0.05)。登革热疫点和非疫点15岁以下中小学生滤纸血样标本中均未检测到抗登革病毒IgG抗体。RT-PCR法未检测到抗体阳性者血清中有登革病毒。结论登革热疫点人群抗登革病毒IgG阳性率明显高于非疫点人群,疫点人群抗体阳性率随年龄增长而上升,提示登革热疫点在登革热疫情后,登革病毒可能在蚊媒体内存在低密度的循环。
Objective To understand the anti-dengue virus antibody level and distribution in healthy population of dengue-endemic areas. Methods In the area of Yuzhu Street, Huangpu District, where the dengue type Ⅱ epidemic broke out in 1986, the serum samples were collected from healthy subjects without clinical symptoms in 2 weeks. Serum anti-dengue IgG antibody was detected by ELISA. The positive rate of anti-dengue virus IgG antibodies in healthy population was compared. The serum of dengue virus was detected by RT-PCR. Results The positive rate of anti-dengue virus IgG was 23.3% in healthy population of dengue epidemic point and 3.8% of anti-dengue virus IgG in healthy population of non-dengue epidemic group, the difference was statistically significant (P <0.01); dengue fever The antibody positive rate of point population was positively correlated with age. The positive rate of antibody in each age group of 0 ~ 20,21 ~ 30,31 ~ 40,41 ~ 50,51 ~ 60,61 ~ 70 and over 70 years were 3.8%, 5.8 %, 9.1%, 26.5%, 27.7%, 32.5% and 35.1%. The difference of the positive rate of anti-dengue virus IgG antibody between the age group of 30 years old and the age group of 31-40 years, the age group of 31-40 years and the age group of 41-60 years, the age group of 41-60 years and the age group of 61 years were respectively Statistically significant (P <0.05). Dengue fever and non-epidemic point of 15-year-old primary and secondary filter paper blood samples were not detected in anti-Dengue virus IgG antibodies. There was no dengue virus in the sera of patients who were positive by RT-PCR. Conclusion The positive rate of anti-dengue virus IgG in dengue fever population was significantly higher than that in non-devastated population. The positive rate of antibody increased with age in dengue population, suggesting dengue virus may exist in mosquito media after dengue fever epidemic Low-density circulation.