为构建一种新型钩体重组亚单位疫苗，采用问号钩体ｒｐＤＪｔ基因的蛋白表达物Ｐ６８免疫动物，对其免疫原性进行研究。结果显示：在体外，Ｐ６８能与其特异性抗血清及赖型钩体死菌苗抗血清呈明显高效价Ａｇ－Ａｂ反应；在体内，Ｐ６８能激起明显的Ｔ淋巴细胞转化，ＴＨ细胞活化，ＩＬ－２，ＩＬ－６活性增加。提示：ｒｐＤＪｔ表达蛋白Ｐ６８能激起机体强有力的Ｔ－Ｂ细胞协同性特异性体液免疫反应，是致病性强毒力钩体重组亚单位疫苗好的候选抗原之一。 In order to construct a new recombinant subunit vaccine, the immunogenicity of the rpDJt gene was tested by immunizing the animals with P68 protein. The results showed that in vitro, P68 was able to react with Ag-Ab with high specific titer antiserum against its specific antiserum and live attenuated leptospira bacterin; in vivo, P68 evoked significant T lymphocyte transformation and TH cell activation, IL-2, IL-6 activity increased. Tip: rpDJt expression of protein P68 can stimulate the body’s strong synergistic T-B cell-specific humoral immune response, is a virulent pathogenic virion recombinant subunit vaccine one of the good candidate antigen.