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目的:探索在大鼠局灶性脑缺血再灌注后神经细胞的再生,研究新生神经细胞的功能。方法:取60只成年大鼠随机分成两组,假手术对照组和脑缺血再灌注动物模型(MCAO)组,各组又分为再灌注3 d、7 d、14 d3个亚组,每组10只大鼠,分别用免疫组化和免疫荧光技术检测新生神经细胞,用流式细胞仪统计各脑区新生神经细胞数。结果:免疫组化结果显示,脑缺血再灌注后在大脑皮层、纹状体、侧脑室区、蛛网膜下腔和海马CA1区都存在Brdu阳性细胞;免疫荧光结果显示新生的神经细胞能分泌Ach和GABA神经递质;流式细胞仪计数统计结果显示在不同的时间点都有新生神经细胞,其中以7 d新生神经细胞数达到高峰。结论:大鼠急性脑缺血再灌注后在损伤的脑组织中能大量再生神经细胞并有少数存活。
Objective: To explore the regeneration of nerve cells after focal cerebral ischemia-reperfusion in rats and to study the function of newborn neurons. Methods: Sixty adult male rats were randomly divided into two groups: sham operation control group and MCAO group. Each group was divided into 3 subgroups: reperfusion 3 d, 7 d and 14 d Groups of 10 rats were detected by immunohistochemistry and immunofluorescence assay of neonatal nerve cells, using flow cytometry brain cells in each area of newborn neurons. Results: The results of immunohistochemistry showed that there were Brdu positive cells in cerebral cortex, striatum, lateral ventricle area, subarachnoid space and hippocampal CA1 area after cerebral ischemia-reperfusion. Immunofluorescence results showed that nascent nerve cells secreted Ach and GABA neurotransmitter. The results of flow cytometry showed that there were newborn neurons at different time points, and the number of newborn neurons peaked on the 7th day. CONCLUSION: After acute cerebral ischemia-reperfusion in rats, a large number of nerve cells can regenerate in the damaged brain tissue with a few surviving.