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目的:比较不同地区及生境中的哈蟆油药材中总多糖的含量,为评价该药材质量提供依据。方法:石油醚脱脂、水回流提取、氯仿萃取,苯酚-硫酸法显色,葡萄糖对照,可见分光光度法测定。结果:哈蟆油药材中总多糖含量,不同地区的以林下产的高于稻田的;同一生境中以黑龙江地区的高于吉林、辽宁的。结论:以哈蟆油药材中总多糖的含量评价其药材质量,与该药材的传统主产区及目前市场定价相吻合。
OBJECTIVE: To compare the content of total polysaccharide in Ruglalpha oil in different regions and habitats, and to provide a basis for evaluating the quality of the medicinal materials. Methods: Petroleum ether degreasing, water reflux extraction, chloroform extraction, phenol - sulfuric acid color, glucose control, visible spectrophotometry. Results: The content of total polysaccharide in Rana oil was higher than that in paddy field in different regions. In the same habitat, the content of total polysaccharide in Heilongjiang was higher than that in Jilin and Liaoning. Conclusion: The content of total polysaccharides in Fructus Rugosae oil to evaluate the quality of medicinal herbs, which is consistent with the traditional main producing areas of the medicinal materials and the current market pricing.