论文部分内容阅读
用柠檬酸提取人类骨胳肌肉膜蛋白抗原(CAE),先按间接血凝法(IHA)测103例重症肌无力(MG)血清中CAE-Ab,其中伴胸腺瘤者阳性率为78.9％(15/19);后改用ELISA方法,检测123例MG,其中伴胸腺瘤者阳性率为82.6％(19/23)。MG有胸腺瘤与无瘤、其他疾病及正常人组对照,抗体滴度相差非常显著(P<0.01)。结果表明CAE-Ab对MG伴胸腺瘤具有显著相关性。本文列举6例,说明临床采用敏感而又特异的CAE-Ab检测方法,可以早期发现胸腺瘤;中年以后MG患者常规检测CAEAb,可避免漏诊;即使瘤体徽小,CT扫描影像分辨不清,也可辅助临床诊断;病程中动态观察抗体滴度,可以发现隐藏着或复发的肿瘤,有助于随访,加强治疗。成批检测CAE-Ab时可出现少数“假阳性”、“假阴性”,诊断必须结合临床,综合分析。
Human skeletal muscle-membrane protein antigen (CAE) was extracted with citric acid. The CAE-Ab in 103 cases of myasthenia gravis (MG) serum was measured by indirect hemagglutination (IHA) method. The positive rate of accompanied with thymoma was 78.9% ( 15/19); ELISA method was used to detect 123 cases of MG, of which the positive rate of thymoma was 82.6% (19/23). MG had thymoma contrasted with tumor-free, other diseases and normal controls, and the antibody titers were significantly different (P<0.01). The results show that CAE-Ab has a significant correlation with MG with thymoma. This article enumerated 6 cases, indicating that the clinical use of sensitive and specific CAE-Ab detection method can be early detection of thymoma; routine detection of CAEAb in middle-aged MG patients can avoid missed diagnosis; even if the tumor emblem is small, CT scan image can not distinguish , Can also assist clinical diagnosis; dynamic observation of antibody titers during the course of the disease, can find hidden or recurrent tumors, help follow-up, strengthen treatment. A small number of “false positives” and “false negatives” may appear when testing CAE-Abs in batches. The diagnosis must be combined with clinical analysis.