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目的观察多烯紫杉醇对SPC-A1肺癌细胞放射增敏作用及其机制。方法①将SPC-A1肺癌细胞分别暴露于多烯紫杉醇24h和48h后给于γ射线照射。照射后培养10天统计不同剂量γ射线照射后的存活分数,以单靶多击模型分析各实验组存活分数在37%时的放射增敏比(SER);②向上述经过多烯紫杉醇处理后的SPC-A1肺癌细胞培养液中加入2′,7′-二氯双氢荧光素二乙酸盐(DCFH-DA)和二氢乙啶(HE),利用流式细胞仪测定活性氧活性。结果1.25×10-10和2.5×10-10mol/L多烯紫杉醇作用于SPC-A1肺癌细胞24h的SER分别为1.10和1.23;作用48h的SER分别为1.36和1.43。1.25×10-10和2.5×10-10mol/L多烯紫杉醇作用于SPC-A1肺癌细胞24h后细胞内活性氧分别为42.59%和49.22%;作用48h后细胞内活性氧分别为51.42%和66.03%;与对照组(21.71%)比较差异有统计学意义(P<0.01)。结论①多烯紫杉醇对体外培养的SPC-A1肺癌细胞具有放射增敏作用;②多烯紫杉醇的放射增敏作用与活性氧有关。
Objective To observe the radiosensitization effect of docetaxel on SPC-A1 lung cancer cells and its mechanism. Methods ① SPC-A1 lung cancer cells were exposed to docetaxel 24h and 48h after irradiation for γ-rays. After 10 days of irradiation, the survival scores of different doses of γ-rays after irradiation were calculated. The single-target multiple-hit model was used to analyze the radio-sensitization ratio (SER) when the survival rate of each experimental group was 37%. ② After the above docetaxel treatment (DCFH-DA) and dihydropyridine (HE) were added into SPC-A1 lung cancer cell culture medium, and the activity of ROS was measured by flow cytometry. Results The SER of 1.25 × 10-10 and 2.5 × 10-10mol / L docetaxel for 24 hours in SPC-A1 lung cancer cells were 1.10 and 1.23, respectively. The SER values at 48h were 1.36 and 1.43.1.25 × 10-10 and 2.5 The intracellular reactive oxygen species (ROS) were 42.59% and 49.22% respectively after treated with docetaxel for 10-10mol / L for 24 hours. The intracellular reactive oxygen species were 51.42% and 66.03% respectively after treated with docetaxel for 48 hours. Compared with the control group (21.71 %) Was statistically significant (P <0.01). Conclusions ① Docetaxel has radiosensitization effect on SPC-A1 lung cancer cells cultured in vitro; ② Radiosensitization of docetaxel is related to active oxygen.