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为了解大麦亲本材料间的遗传差异及农艺性状特征,利用46对SSR引物对61份大麦材料进行遗传多样性分析,并鉴定了其田间农艺性状。结果表明,株高最高和最矮的材料分别为苏盐0014和Z122V032W;穗长最长和最短者分别为阿恩特13和富士二条;BYT-CRA3的穗粒数最多。不同农艺性状的相关性分析表明,株高和第二节间长,第一节间长和第二节间长,穗长和穗粒数均具有极显著的相关性。SSR分析结果表明,46对SSR引物在61份材料中共检测到117个等位变异,单个引物可检测到1～5个等位变异,平均每个引物可检测到2.5个。聚类分析结果表明,参试材料遗传相似系数(GS)为0.572 6～0.954 4,平均值为0.753 0。在GS为0.726 0处,61份材料可聚为5大类。大多数国内材料被聚为一类,国外材料与国内材料遗传差异较大。
In order to understand the genetic differences and agronomic traits among the parents, 46 SSR primers were used to analyze the genetic diversity of 61 barley cultivars and their agronomic traits were identified. The results showed that the highest and the lowest plant height were Suyu 0014 and Z122V032W, respectively. The longest and shortest spike length were Arnd 13 and Fuji 2, respectively. BYT-CRA3 had the most spikelets. The correlation analysis of different agronomic traits showed that the plant height and the second internode length, the first internode length and the second internode length, the spike length and the number of grains per spike had a very significant correlation. The results of SSR analysis showed that 117 alleles were detected in 61 SSR primers and 1 to 5 alleles were detected in a single primer, with an average of 2.5 alleles per primer. Cluster analysis showed that the genetic similarity coefficient (GS) of the tested materials was 0.572 6 ~ 0.954 4 with an average of 0.753 0. At GS 0.726 0, 61 materials can be grouped into five broad categories. Most domestic materials are grouped into a category, foreign materials and domestic material genetic differences.