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细胞表面的力学性质会随着细胞所处环境的不同而发生改变,它的变化间接反映出胞内复杂的生理过程。原子力显微镜(atomic force microscope,AFM)能以高的灵敏度和分辨率检测活体细胞,通过利用赫兹模型分析力曲线可以获得细胞的弹性信息。本文简介了原子力显微镜的工作原理与工作模式,着重介绍利用AFM力曲线检测细胞弹性的方法及其在细胞运动、细胞骨架、细胞黏附、细胞病理等方面的应用成果,表明AFM已经成为细胞弹性研究中十分重要的显微技术。
The mechanical properties of the cell surface will change with the cell environment, and its changes indirectly reflect the intracellular complex physiological process. Atomic force microscope (AFM) can detect living cells with high sensitivity and resolution. The elasticity of cells can be obtained by analyzing the force curve using the Hertzian model. In this paper, the working principle and working mode of AFM are introduced. The method of using AFM force curve to detect cell elasticity and its application in cell motility, cytoskeleton, cell adhesion and cell pathology are introduced. It is shown that AFM has become a research topic of cell elasticity In the very important microscopy.