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目的探讨使用甲壳素衍生物—羧化壳聚糖即刻分离制备血小板的可行性,寻找血液成分分离新方向。方法随机选取大连地区献血者40名,当日成功献血时同步采集全血10 m L,分成实验组,自然沉降对照组和离心对照组进行实验。从阿拉丁试剂公司购买水溶性壳聚糖,用血液保存液Ⅱ配制成(0.25~2.0)mg/m L不同浓度溶液,按1∶4比例加入到2 m L全血中,静置4 h,观察血液分离速度,计数血浆中红细胞,白细胞及血小板,观察血小板聚集现象及红细胞形态变化;在最适壳聚糖添加浓度的红细胞中加入适量红细胞保存液予以4℃保存,35 d保存期内检测溶血率。结果加入1.0~2.0 mg/m L浓度壳聚糖的实验组血液分离速度均明显快于自然沉降对照组,血浆中均仅存微量红细胞,富血小板,35d时无明显溶血,同离心对照组。结论壳聚糖可以用于采血后即刻分离富血小板血浆。
Objective To explore the feasibility of using chitin derivative - carboxylated chitosan to prepare platelets immediately and to find a new direction of separation of blood components. Methods Forty blood donors were randomly selected in Dalian. The blood samples were collected simultaneously on the day of successful blood donation, and were divided into experimental group, natural sedimentation control group and centrifugal control group. Water-soluble chitosan was purchased from Aladdin Reagent Company, and different concentrations of (0.25-2.0) mg / m L solution were prepared from blood preservation solution II and added to 2 mL of whole blood at a ratio of 1: 4. After standing for 4 h , Observe the speed of blood separation, count red blood cells, white blood cells and platelets in plasma, observe platelet aggregation phenomenon and red blood cell morphological changes; add appropriate amount of erythrocytes in the optimal concentration of chitosan preservation solution to be stored at 4 ℃, 35 d preservation period Hemolysis rate was measured. Results The blood separation speed of the chitosan group with 1.0-2.0 mg / mL concentration of chitosan was significantly faster than that of the natural sedimentation control group. There were only trace erythrocytes and platelet-rich plasma in the plasma. There was no obvious hemolysis on the 35th day in the control group. Conclusion Chitosan can be used to separate platelet-rich plasma immediately after blood sampling.