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目的:体外模拟腹腔镜手术二氧化碳气腹方法,探讨二氧化碳(CO2)环境对人宫颈癌Hela细胞黏附和侵袭能力的影响。方法:培养人宫颈癌Hela细胞株,模拟CO2气腹环境12 mmHg气压处理4 h,以培养于常规条件下人宫颈癌Hela细胞为对照,通过MTT法检测细胞生长变化,通过细胞基质黏附试验检测细胞基质黏附能力改变,应用免疫组织化学方法检测人宫颈癌Hela细胞中E-钙黏素(E-cadherin)和基质金属蛋白酶-9(matrix metalloproteinase-9,MMP-9)的表达变化。结果:与对照组相比,CO2气腹处理组生长曲线上移(P<0.05)、细胞基质黏附能力增强(P<0.05)、E-钙黏素表达减少(P<0.05)、基质金属蛋白酶-9表达增加(P<0.05)。结论:CO2气腹环境增加宫颈癌侵袭和转移的风险,其机制之一可能与人宫颈癌Hela细胞的E-钙黏素的表达降低、基质金属蛋白酶-9的表达增加有关。
Objective: To evaluate the effects of carbon dioxide (CO2) environment on the adhesion and invasion of human cervical carcinoma Hela cells in vitro. Methods: Human cervical cancer Hela cells were cultured and treated with 12 mmHg of CO2 pneumoperitoneum for 4 h. The cultured human cervical cancer Hela cells were used as control. The cell growth was detected by MTT assay. The change of cell adhesion was observed. The expression of E-cadherin and matrix metalloproteinase-9 (MMP-9) in human cervical carcinoma Hela cells was detected by immunohistochemistry. Results: Compared with the control group, the growth curve of CO2 pneumoperitoneum was up-shifted (P <0.05), the adhesion of cell matrix was enhanced (P <0.05), the expression of E-cadherin was decreased -9 expression increased (P <0.05). CONCLUSION: CO2 pneumoperitoneum increases the risk of cervical cancer invasion and metastasis. One of the mechanisms may be related to the decrease of E-cadherin expression and the increase of matrix metalloproteinase-9 in human cervical cancer Hela cells.