本研究应用凝胶迁移法检测局灶脑缺血区中Ｆｏｓ与Ｊｕｎ基因的蛋白产物形成异源二聚体复合物—转录因子ＡＰ１（ａｃｔｉｖａｔｏｒｐｒｏｔｅｉｎ１）的ＤＮＡ结合活性。缺血３０ｍｉｎ或９０ｍｉｎ伴随再灌流４ｈ，结果表明ＡＰ１结合活性增加。提高的ＤＮＡ结合活性持续２４ｈ。以上结果说明提高转录因子ＡＰ１的表达水平在缺血病变的基因调节改变中起一定作用 In this study, gel electrophoresis was used to detect the DNA binding activity of the protein product of Fos and Jun genes in the focal cerebral ischemic area to form the heterodimer complex AP-1 (activator protein 1). Ischemia 30min or 90min with reperfusion 4h, the results show that AP 1 binding activity increased. Increased DNA binding activity lasts 24 h. The above results show that increasing the expression level of transcription factor AP 1 plays a role in the genetic regulation of ischemic lesions