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[期刊论文] 作者:LIJi-chang,TONGGuang-zhi,QIUHu, 来源:东北农业大学学报:英文版 年份:2003
By means of PCR,the gene encoding gD of bovine herpesvirus-1 (BHV-1) strain Luojing was amplified,cloned and sequenced.The nucleotide sequence of this gD gene w...
[期刊论文] 作者:LIJi-chang,TONGGuang-zhi,QIUHu, 来源:东北农业大学学报:英文版 年份:2004
The gene encoding gD of isolate Luojing of infectious bovine rhinotracheitis virus (IBRV)was amplified,sequenced, and cloned into plasmid pcDNA 3.1, resulting i...
[期刊论文] 作者:CHIYu-jie,YANGQian,LIJi-chang, 来源:东北农业大学学报:英文版 年份:2003
An about 1.40 Kb target gene fragment was yielded by PCR amplification with the plasmid pRB 129,which was identified by restriction enzyme digestion that the PC...
[会议论文] 作者:TIANLi[1]田力[2]LIJi-chang[1]李继昌[2]ZHAOGuo-qiang[1]赵国强[2]CHENKui-sheng[1]陈奎生[2], 来源:2006年郑州大学博士生学术论坛 年份:2006
  目的:构建TIMP-1小干扰RNA真核表达载体并观察其对肝星形细胞株TIMP-1 mRNA表达的作用.方法:根据siRNA设计原则,在TIMP-1 mRNA序列中选择2个特异性靶序列(447-465nt、552-5...
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