expressi相关论文
针对白细胞介素18（IL-18）基因和本实验室分离的PRRSVJL/07/SW毒株全基因序列,分别设计了2对特异性引物,扩增出IL-18基因和ORF6基因。......
用PCR方法扩增副猪嗜血杆菌的外膜蛋白ompP2基因,序列测定结果表明扩增片段全长1 188bp。将扩增片段插入表达载体pET-32a,转化BL21......
【目的】钙结合蛋白作为钙信号传导途径的组分,通过与钙离子结合实现其生物学功能,调控细胞信号传导和细胞生命周期等过程,且钙结......
目的利用同种异体小鼠异位气管移植模型，研究NF—κB是否激活MCP-1基因参与闭塞性细支气管炎（OB），探讨OB的发病机制。方法将小鼠异位气......
Determining how function evolves following gene duplication is necessary for understanding gene expansion.Transcription ......
Nucleopolyhedrovirus (NPV) is divided into Group Ⅰ and Group Ⅱ based on the phylogenetic analysis. It has been reporte......
AIM: To study the expression patt of ETS2 (erythroblastosisvirus oncogene homolog 2) in human esophageal squamouscell ca......
MORE than a year ago, a ceremony was held to celebrate the opening of the first senior high school for girls in Beijing......
Neuron-specific enolase(NSE) is a tumor marker for neuroblastoma and also one of the mostsensitive and specific marker ......
Single amino acid mutation of SR-BI decreases infectivity of hepatitis C virus derived from cell cul
AIM To investigate the effect of a single amino acid mutation in human class B scavenger receptor I(SR-BI) on the infect......
AIM:To construct a DNA vaccine encoding human alpha-fetoprotein(hAFP)/heat shock protein 70(HSP70),and tostudy its abil......
Study on Transplanting Germ Like Cells Differentiated from Embryonic Stem Cell into Mouse Seminifero
Objective To investigate whether germ like cells isolated from embryoid body formed by mouse embryonic stem cells could ......
【Abstract】Chinese characteristic vocabulary is a tool for expressing specific things. Language translators studied cor......
Ideal access structures admit ideal secret sharing schemes where the shares have the minimal size.As multipartite access......
【目的】研究苜蓿夜蛾Heliothis viriplaca保幼激素酯酶（Juvenile hormone esterase,JHE）的功能和作用机理。【方法】本试验提取苜蓿......
Molecular cloning and characterization of a lipid transfer protein gene (PsLTP1) from Pinus sylvestr
该文从挂篮荷载计算、施工流程、支座及临时固结施工、挂篮安装及试验、合拢段施工、模板制作安装、钢筋安装、混凝土的浇筑及养生......
为探究吕家坨井田地质构造格局,根据钻孔勘探资料,采用分形理论和趋势面分析方法,研究了井田7......
The first intron (EPI) of rice 5-enolpyruvylshikimate 3-phosphate synthase gene was isolated by PCR from one clone with ......
Molecular Cloning and Bacterial Expression of Germacrene A Synthase cDNA from Crepidiastrum sonchifo
为探究吕家坨井田地质构造格局,根据钻孔勘探资料,采用分形理论和趋势面分析方法,研究了井田7......
AIM To investigate the relationship between the expression of p16 gene and the gastric carcinogenesis,depth of invasion ......
Two cDNAs of plastid division gene NtFtsZ1-1 and NtFtsZ1-2 are isolated from Nicotiana tabacum by RT-PCR and rapid ampli......
The rotational dispersion coefficient of the fiber in the turbulent shear flow of fiber suspension was studied theoretic......
目的：构建肺炎链球菌StkP／PhpP信号偶联中磷酸酶编码基因phpP原核表达系统，了解表达产物rPhpP磷酸酶活性及类型。方法采用PCR扩增肺炎......
Molecular Cloning and Bacterial Expression of Germacrene A Synthase cDNA from Crepidiastrum sonchifo
Germacrene A synthase(GAS) catalyzes the biosynthesis of germacrene A, which is a key precursor for sesquiterpene lacton......
目的 探讨膜型基质金属蛋白酶（memberane-type matrix metalloproteinases,MT-MMPs）在内皮细胞中的作用。方法 用Clustal W软件（http：/......
采用RT-PCR技术从美洲大蠊中扩增出变应原Cr-PⅡ基因，并将其克隆至pMDl8．T载体中。经序列测定和分析证实，所克隆的片段古有长度为1185......
本研究对破伤风毒素C片段进行了基因克隆、重组表达、蛋白纯化和免疫原性分析。应用PCR技术直接从破伤风梭菌64008菌株基因组中扩......
根据副猪嗜血杆菌(Haemophilus parasuis,Hps)外膜蛋白(outer membrane proteins,Omp)P2基因序列(GenBank登录号:FJ685754.1)设计......
目的构建N端融合有Gal4DBD的hDaxx及6种删除突变体(△hDaxx)真核细胞表达质粒,并在HT1080细胞中表达.方法将hDaxx及△hDaxx cDNA酶......
利用PCR和TA克隆方法扩增和克隆得到了恶臭假单胞菌Pseudomonas putida S1的海藻糖合成酶基因treS。对其进行序列分析表明，其编码区......
目的探讨鼻咽上皮癌变过程中周期蛋白CyclinD1与抑癌基因p21（wild—type p53 activated fragment 1，p21wAF1）蛋白的表达及其意义。方......
目的研究TIMP-1、MMP-2和MMP-9在大肠癌组织中表达的临床意义。方法采用免疫组化SP法检测50例大肠癌、10例正常大肠黏膜组织中TIMP......
过氧化物酶体增殖物激活受体（PPARs）是一类配体依赖的核转录因子，属核受体超家族成员。自从PPARs于1990年被发现以来，研究人员对啮齿动......
近距离工作条件下的位移，可用无盲区的单光纤传感器来测量，针对这种情况，作者导出了能量耦合效率的普适解析表达式、并与目前普遍采用......
【目的】探索中间锦鸡儿基因组中FAD2基因的拷贝数,分析不同拷贝的表达模式,以期揭示不同拷贝在植物体内的功能分工。【方法】根据......
目的：研究同型半胱氨酸诱导基因（HCY-2）在发育不同时期的正常人胚气管和肺中的表达,揭示HCY-2基因在呼吸系统发育中的作用。方法：取正......
海栖热袍菌Thermotoga maritima是嗜极端高温的厌氧细菌，其产生的极耐热葡聚糖酶具有很好的热稳定性和潜在的可观工业用途。但嗜高......
大豆疫病严重影响我国及世界各国的农业生产,为探讨多聚半乳糖醛酸酶在大豆疫霉菌致病过程中的作用,采用PCR的方法从大豆疫霉菌中......
目的检测上皮性卵巢癌(EOC)患者肿瘤组织以及血清microRNA-15a/b(miR-15a/b)表达水平,并探讨其临床意义。方法回顾性选取30例卵巢......
期刊
通过DNA重组技术,将克隆到的MDPV-Q和MDPV-26的VP2基因片段,经两种限制性核酸内切酶SaclI和Kp-nl的酶切,将酶切产物再克隆到表达载......
本研究以伪狂犬病病毒Ea株基因组DNA为模板，PCR扩增含UL18全长基因的片段并克隆至pMD18-T载体，采用双脱氧末端终止法测序。序列分析......
选用３９ＫＤａ的钩端螺旋体外膜蛋白免疫豚鼠并施予艾灸，研究艾灸对伤害性刺激所和延髓中枢原癌基因ｃ－ｆｏｓ表达的影响。结果表明：灸加ＯｍｐＬ３９，单用ＯｍｐＬ３９和单纯艾灸......
Acute exposure to high-peak-power pulsed microwaves affecting the histamine H3 receptor expression i
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关系词在现代汉语复句领域起着重要的作用,是汉语语法、语义研究中的重要内容,复句关系词的计算机自动识别是一个非常困难的研究课......
淀粉分支酶可增加淀粉的α-1，6-糖苷键，从而改善淀粉的理化特性和生理功能。本文以可胞内表达Geobacillus thermoglucosidans淀粉分......
Effective Penetration of Cell-permeable Peptide Mimic of Tyrosine Residue 654 Domain of β-catenin in
Phosphorylation of β-catenin tyrosine residue 654 plays an important role in the epithelial to myofibroblast transition......
目的 克隆镰形扇头蜱肌钙蛋白Ⅰ（TnⅠ）的cDNA并进行表达及鉴定。方法 用RT-PCR方法从镰形扇头蜱总RNA中克隆编码肌钙蛋白Ⅰ的cDNA片......
Molecular cloning and characterization of a lipid transfer protein gene (PsLTP1) from Pinus sylvestr
Plant nonspecific lipid transfer proteins (nsLTPs) are widely distributed through plant kingdom and are characterized by......
采用3（情绪调节策略：认知重评、表达抑制、无策略）×3（英语词汇性质：积极、中性、消极）的混合实验设计,考察在诱发负性情绪条件下,采......
笔者对《实用翻译教程》一书中的部分观点（用词结构不得更改一说）和译文特别是改译等提出了商榷，并举例予以分析。......
