在对１２个以ＲＴ－ＰＣＲ扩增后的ｃＤＮＡ片段进行克隆的工作中，讨论了影响克隆效率的几个因素，并对原有的部分克隆步骤作了优化，取得了对适当长度基因片段进......

[Objective] To clone and express canine IL-2 gene and thus to provide theoretical support for the development of novel i......

利用ＴａｑＤＮＡ聚合酶的无模板延伸活性制备了可直接克隆ＰＣＲ产物Ｔ载体，苗对猪瘟病毒ＨＣＬＶ株的ＲＴ－ＰＣＲ产物进行了克隆。......

本文采用DAS-ELISA、RT-PCR方法从隔离试种的进口荷兰百合中检出百合无症病毒,并通过对RT-PCR产物进行序列分析验证,确认进口的百......

[Objective] To express lactate dehydrogenase C (LDH-C) gene in prokaryotic system and then purify the recombinant protei......