cDNAcloning相关论文
Identification of a GH3 homologue in Echinochloa crus-galli (L.) P.Beauv.(Barnyard grass), and its d
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Molecular Cloning, Characterization and Expression of a Transient Receptor Potential Gene from Bemis
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Identification and characterization of the first reptilian CD9, and its expression analysis in respo
In this study, a CD9 homologue in a reptile, Chinese soft-shelled turtle, has been cloned and identified for the first t......
cDNA, genomic sequence cloning and Overexpression of Ribosomal Protein S16 Gene (RPS16) from the Gia
RPS16 of eukaryote is a component of the 40S small ribosomal subunit encoded by RPS16 gene and is also a homolog of prok......
Cloning and characterization of three cDNAs encoding different GnRHs in the brain of spotted halibut
Spotted halibut is a large cold-water pleuronectiform fish inhabiting coastal areas of China,Japan and Korea.The tasty f......
NgRDR1,a Novel RNA-Dependent RNA Polymerase Isolated from Nicotiana glutinosa, Was Involved in Bioti
The RNA-dependent RNA polymerases (RDRs) play a key role in RNA silencing,heterochromatin formation and natural gene reg......
A Novel igf3 Gene in Common Carp(Cyprinus carpio):Evidence for Its Role in Regulating Gonadal Develo
Since the insulin-like growth factor 3(igf3)gene was recently discovered in fish ovary,its function in the gonads has re......
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Gene cloning and expression of a carboxylesterase from the striped stem borer, Chilo suppressalis (W
Carboxylesterases (CaEs) are a large and diverse class of enzymes implicated in xenobiotic detoxification, hormone and p......
用ＲＴ－ＰＣＲ方法快速克隆了Ｗｉｓｔａｒ大鼠脑神经元特异性烯醇化酶的ｃＤＮＡ，将此包括编码全长ＮＳＥ４３３个氨基酸的ＤＮＡ片段重组入ｐＵＣ质粒，并用ＰＣＲ方法测定了全部顺序，经重复实验，发现......
使用ＲＴ－ＰＣＲ方法克隆了Ｗｉｓｔａｒ大鼠脑α１型甲状腺激素受体的ｃＤＮＡ，得到包含起始及终止密码子共１２３３ｂｐ、编码４０９个氨基酸的受体全长编码离列，酶切分析后，将此特异ＤＮＡ片段......
Human vascular endothelial growth factor (VEGF) cDNA was amplified by nested polymerase chain reaction method from the H......
We isolated a novel lectin (AHL) from Artocarpus hypargyreus Hance and showed its immunomodulatory activities.In this st......
从中国汉族人胎肝组织提取ＲＮＡ，以ＲＴ－ＰＣＲ方法获得了编码含号顺序的全长甘露聚糖结合凝集素肽链的ｃＤＮＡ片段，将其与ｐＧＥＭ－Ｔ载体连接，转化大肠杆菌ＴＧ１，......
目的克隆人白介素 - 18结合蛋白 (h IL- 18BP)的 c DNA,以研究其结构与功能的关系。方法从中国汉族人的胎盘组织中提取总 RNA,以 R......
目的 克隆并原核表达α４亚基笥段。方法 从ＩＬ－６细胞系中提取总ＲＮＡ，以ＲＴ－ＰＣＲ方法分两段扩增人整素分子α４亚基片段２．０ｋｂ ｃＤＮＡ，将两片段连接，并将其中的１．７ｋｂ基因片......
全长cDNA的获得是基因克隆的重要内容，也是基因组研究的重要内容之一。cDNA末端快速扩增技术（RACE）是获得全长cDNA的主要辅助手段之一......
