clon相关论文
Avian influenza remains a threat to human wellbeing. Hypochlorite derivatives are commonly used as disinfectants to prev......
AIM:To construct human eosinophil-derived neurotoxin(hEDN) and HBV core protein (HBVc) eukaryotic fusionexpression vect......
AIM:To explore the expression of heparanase mRNA andpoint mutation in hepatocellular carcinoma(HCC).METHODS:Reverse tra......
Prevalence of clonorchiasis in patients with gastrointestinal disease: A Korean nationwide multicent
AIM: To investigate prevalence of Clonorchis sinensis in patients with gastrointestinal symptoms, and the relation of th......
Dunaliella salina, a halotolerant unicellular green alga without a rigid cell wall, can live in salinities ranging from ......
AIM:To identify and compare the profile of Ca2+ channel subunit expression in INS-1 and rat pancreatic β cells. METHODS......
CLONING OF cDNA CODING FOR CARBAMYL PHOSPHATE SYNTHETASE I AND CHANGES IN LEVELS OF CPS_1 mRNA DURIN
cDNA coding for carbamyl phosphate synthetase I was cloned from recombinant plasmid with insertcomplementary to the mRN......
A novel,truncated human bone morphogenetic protein-2 :construction,expression ,functions and clinica
Introduction As a member of the bone morphogenetic protein (BMP) family,BMP-2 playsimportant roles not only in bone ......
Objective: To obtain the complete β-actin gene from Aedes albopictus. Methods: Total RNA was extracted from C6/36 cells......
Construction of recombinant attenuated Salmonella typhimurium DNA vaccine expressing H pylori ureB a
AIM: To construct a recombinant live attenuated Salm-onella typhimurium DNA vaccine encoding H pylori ureB gene and mous......
Objective To develop directly molecular evolution of nitrite oxido-reductase using DNA-shuffling technique because nitro......
Cloning,Tissue Distribution,and Transmembrane Orientation of the Olfactory Co-Receptor Orco from Two
In insects,the sense of smell is mainly mediated by olfactory receptors(Ors).Olfactory co-receptor(Orco),which is coexpr......
Characterization of cancer stem-like cells in the side population cells of human gastric cancer cell
Objective:Side population(SP) cells may play a crucial role in tumorigenesis and the recurrence of cancer.Many kinds of ......
Recent Advances on Key Enzyme Genes in Metabolic Pathways Related to the Synthesis of Aroma Compound
This article summarized three main kinds of metabolic pathways related to the synthesis of aroma compounds in plants,con......
Screening of Differently Expressed Genes in Human Prostate Cancer Cell Lines with Different Metastas
In order to screen the genes differentially expressed in two human prostate cancer cells with different metastasis poten......
Screening of Differently Expressed Genes in Human Prostate Cancer Cell Lines with Different Metastas
In order to screen the genes differentially expressed in two human prostate cancer cells with different metastasis poten......
[目的]对五指山小型猪脱嘌呤嘧啶核酸内切酶cDNA进行克隆和生物信息学分析.[方法]以构建的五指山小型猪外周血白细胞cDNA文库为材......
利用前期筛选出的一些与母猪妊娠相关的有价值的新候选基因,选取其中一个位于网络顶端的EST序列（CK455976）,根据此EST序列设计引物,......
本研究通过RT-PCR技术从人胎盘组织中扩增出长为841bp的胰岛素样生长因子-ⅡcDNA,PCR产物经凝胶回收纯化后,克隆在PGEM-T载体的T位......
以油菜(Brassica napus)基因组DNA为模板,通过设计一对特异性引物,利用多聚酶链式反应(PCR)扩增获得oleosin基因片段.将其克隆到E.......
利用同源克隆技术和cDNA末端快速扩增（RACE）技术克隆大黄鱼胞质型磷脂酶A2（cPLA2）基因的cDNA全长。此外,应用实时定量PCR法检测不同日......
根据GenBank中猪Ⅱ型圆环病毒(PCV-2)基因序列,设计两对特异性引物.将PCV-2广东分离株(GD1株)用PK15细胞系培养,从细胞培养物中提......
依据NCBI所登录的鸡传染性支气管炎病毒的M基因序列设计了一对引物,应用TRIzol试剂盒对8个肾型鸡传染性支气管炎病毒陕西地方分离......
在巴山木竹(Arundinaria fargesii E.G.Camus)混交林中采用邻接格子样方法、样线株间距法取样,研究了巴山木竹无性系种群分布格局,......
提出了一种免疫克隆选择算法与模糊C-均值聚类算法相结合的混合聚类算法，是一种有监督学习和无监督学习结合的算法。该算法首先用克......
根据GenBank发表的序列（L33180）设计引物，利用PCR技术扩增了编码家蚕核型多角体病毒（Bombyxmori Nucleopolyhedrovirus，BmNPV）ORF75基因......
目的分析比格犬下丘脑-垂体-性腺轴,雌二醇β受体剪切异构体的存在情况。方法根据NCBI数据库上的比格犬雌二醇受体的基因序列,设计......
目的：克隆犬胰岛素样生长因子(IGF—Ⅰ)编码区基因，构建重组真核表达载体。方法：从犬左心室组织中提取总RNA，通过RT—PCR获取IGF—1 cD......
研究以母竹无性苗和Ⅰ级、Ⅱ级毛竹实生苗为试材,探讨不同竹苗类型的造林效果.造林第3年结果表明,毛竹实生苗林分在新竹数、地径、......
神经干细胞在治疗中枢神经系统退形性病变中发挥重要作用，但在其移植治疗中也面临着一些问题，例如伦理问题及技术问题。如何合理地引......
采用随机扩增多态性DNA（RAPD）技术，对中国对虾“黄海1号”快速生长的第6代群体同一养殖池中生长性状发生分离的两个极端群体、海捕的......
【目的】克隆广西三黄鸡过氧化物酶体增殖物激活受体γ（PPARγ）基因，建立该基因的实时荧光定量PCR，为后续开展广西三黄鸡PPARγ基因组......
【目的】研究广西巴马小型猪单核细胞趋化蛋白-1(MCP-1)基因CDs区的序列特点,为建立广西巴马小型猪动脉粥样硬化模型及揭示动脉粥......
在种群水平上系统研究了不同放牧强度对内蒙古冷蒿小禾草退化草原星毛委陵菜（Potentilla acaulis)种群克隆生长，有性生殖的影响，结果......
为了克隆表达猪I型干扰素受体Ⅱ胞外域（IFN2EC）基因，试验采用RT-PCR技术从健康仔猪肺巨噬细胞中扩增出猪I型干扰素受体Ⅱ胞外域基因的......
血吸虫的生长发育过程中,其抗原表达呈现明显的期别差异性.利用分子生物学技术,从期别差异表达的功能基因中筛选有意义的功能蛋白,......
通过构建斜带石斑鱼垂体cDNA文库,克隆了其生长激素(GH)全长cDNA.斜带石斑鱼GH全长cDNA为955bp,编码的多肽为204 aa.应用PCR方法把......
采用同源克隆及基因组步移的方法，分离克隆了牙鲆肌肉生长抑制素（MSTN）基因。经过序列分析及cDNA验证，牙鲆MSTN基因具有3个外显子和2个......
利用逆转录聚合酶链式反应（RT-PCR）和cDNA末端快速扩增（RACE）等方法,克隆获得剑尾鱼卵黄蛋白原C（Vg C）基因的全长cDNA序列。剑尾鱼Vg C基......
无乳链球菌是引起奶牛乳房炎的常见病原微生物之一,其表面蛋白作为一种高免疫原性的生物大分子,具有较高的种内特异性,是理想的候......
由于对等网络的动态不确定性和任务调度本身的复杂性,使得任务调度过程中的节点搜索与负载平衡等问题很难得到有效解决。为此,应用......
目的：从结核分枝杆菌H37Ra菌株克隆早期分泌性抗原靶6（ESAT6）基因并进行序列分析。方法：以结核分枝杆菌H37Ra菌株基因组DNA为模版,用PC......
【目的】鹅豁眼性状呈隐性伴性遗传,其遗传基础有待揭示。基因FRAS-related extracellular matrix 1（FREM1）编码区的一些隐性突变导......
[目的]为今后深入了解茉莉酸诱导橡胶树乳管分化的分子机理奠定基础。[方法]根据bHLH转录因子基因保守区设计引物，以橡胶树树皮RNA......
[目的]为香蕉钙调神经磷酸酶B亚基样蛋白基因MaCBL1调控机理的研究奠定基础。[方法]根据在香蕉果实抑制缩减文库中获得的一个CBL基......
[目的]克隆尼罗罗非鱼(Oreochromis niloticus)CD247基因(GenBank登录号:MF975639;On-CD247),分析On-CD247的生物信息学特征及其在......
本实验在新鲜分离大鼠背根神经节（ＤＲＧ）细胞上应用全细胞膜片的箝记录研究贤上腺素α２－受体激动剂可乐定（ｃｌｏｎｉｄｉｎｅ）对ＧＡＢＡ－激活电流的调制作用。发现缘大多......
用RT-PCR一步法对云南省不同禽类(鸡、鸽子)3株禽I型副粘病毒F基因进行扩增和克隆,并对其f基因片段核苷酸序列进行分析,结果表明,......
Cloning and Expressing of a Gene Encoding Cytosolic Copper/Zinc Superoxide Dismutase in the Upland C
In this study, a gene encoding a superoxide dismutase (SOD) was cloned from senescent leaves of cotton (Gossypium hirsut......
