目的：构建ｐＢＶ－ＢＰＩ６００－Ｆｃγ１７００重组表达载体，转化大肠杆菌ＤＨ５α，诱导表达ＢＰＩ２３－Ｆｃγ１抗菌重组蛋白。方法：采用ＲＴ－ＰＣＲ技术，从ＨＬ－６０细胞和正常人白细胞ｍＲＮＡ中扩增编码ＢＰＩ２３和ＩｇＧ１Ｆｃ（Ｆｃγ１）的基因；通过连接......

Objective. To construct pBV-BPI600-Fcγ 1700 recombinant expression vector, to transform it into Escherichia coli DH5α ......