【摘 要】
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In this paper, ultra performance liquid chromatography/quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS) and the MetaboLynxTM software combined with mass defect filtering together were appli
【机 构】
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Jiangsu Key Laboratory for High Technology Research of TCM Formulae, Nanjing University of Chinese M
【出 处】
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第十届全国药物和化学异物代谢学术会议暨第三届国际ISSX/CSSX联合学术会议
论文部分内容阅读
In this paper, ultra performance liquid chromatography/quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS) and the MetaboLynxTM software combined with mass defect filtering together were applied to identity the metabolites of isoquereitrin by the intestinal mixture bacteria and ninety six isolated strains from human feces.The human incubated samples collected 72 h in the anaerobic incubator and extracted with ethyl acetate were analyzed by UPLC-Q-TOF/MS within 10 min.A total of parent competent and 5 metabolites were identified by eight isolated strains including Bacillus sp.17, Veillonella sp.23, 32 and Bacteroides sp.40, 41,-56, 75, 88 in vitro.The results indicated that quercetin, acetylated isoquereitrin, dehydroxylated isoquercitrin, hydroxylated quercetin,hydroxymethylated quercetin were the major metabolites of isoquercitrin.Furthermore, a possible metabolism pathway on the biotransformation of isoquercitrin is established in intestinal flora.This study will be helpful for understanding the metabolic rout of isoquercitrin and the role of different intestinal bacteria on the metabolism of natural compounds.
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