基因编码非天然氨基酸扩展荧光蛋白发色团

来源 :第八届全国化学生物学学术会议 | 被引量 : 0次 | 上传用户：b999123

【摘要】

：

通过扩展基因密码子,实现了具有金属结合能力的非天然氨基酸8-羟基喹啉丙氨酸(HqAla)(Cu2+ KD=0.1 fM)在活细胞中的编码.在sfGFP,sfYFP,PsmOrange及eqFP650荧光蛋白的发色团

【作者】

：

刘晓红王江云

【机构】

：

中国科学院生物物理研究所,非编码核酸重点实验室北京市朝阳区大屯路15号,100101

【出处】

：

第八届全国化学生物学学术会议

【发表日期】

：

2013年9期

【关键词】

：

基因编码非天然氨基酸 HqAla 荧光蛋白发色团扩展

下载到本地 , 更方便阅读

下载此文赞助VIP

声明 : 本文档内容版权归属内容提供方 , 如果您对本文有版权争议 , 可与客服联系进行内容授权或下架

论文部分内容阅读

通过扩展基因密码子,实现了具有金属结合能力的非天然氨基酸8-羟基喹啉丙氨酸(HqAla)(Cu2+ KD=0.1 fM)在活细胞中的编码.在sfGFP,sfYFP,PsmOrange及eqFP650荧光蛋白的发色团中心编码HqAla,首次发现以上荧光蛋白的最大发射波长均红移30nm左右.且eqFP650-67-HQAla蛋白的最大发射波长红移至680 nm,是迄今为止报道的类绿色荧光蛋白中远红外区的最大发射波长.

其他文献

选择性检测谷胱甘肽的比率式荧光化学传感器

含巯基的生物小分子,如半胱氨酸(Cys)、同型半胱氨酸(Hcy)、谷胱甘肽(GSH),在维持生物体系氧化还原平衡方面发挥着重要作用.由于含巯基的生物小分子结构和反应活性的相似性,

会议

巯基化合物选择性检测BODIPY

Ratiometric Fluorescence Imaging of Lysosomal Zn2+ Release under Oxidative Stress in Neural Stem Cel

Zinc dyshomeostasis is a major mechanism of neuronal death, which is involved in many different neuropathological conditions.Lysosomal membrane permeabilisation

会议

Zn2+Cd2+fluorescence selectivitylysosomalneural stem cells

Activity-based Chemical Probes Development and Applications

Activity-based chemical probes (ABPs) have gained significant interests in both chemical biology and drug discovery research.Utilizing this type of small molecu

会议

activity-based probeproteasekinase

Specific binding and inhibition of 6-benzylaminopurine to catalase multiple spectroscopic methods co

The interaction and inhibition of 6-benzylaminopurine (6-BA) to beef liver catalase (BLC) have been investigated systematically by using spectroscopic and molec

会议

catalase6-benzylaminopurineinteractioninhibitiondocking

FAD-Ubiquinone Mediated Electron Transfer in Yeast NADH-Ubiquinone Oxidoreductase Revealed using Ele

Electron transport chain, or the respiratory chain, transports the electron from metabolic process derived NADH to the oxygen molecules, and is the most importa

会议

Electron Transportype-Ⅱ NADH DehydrogenaseElectron Spin ResonancePower Satura

Design, synthesis and biological evaluation of Thiazolo[3,2-a]pyrimidin-3-one derivatives as novel i

Staphylococcus epidermidis has become a significant pathogen causing infections due to biofilm formation on surfaces of indwelling medical devices.Biofilms asso

会议

thiazolo[32-a]pyrimidin-3-oneantibacterial activityantibiofilm activitystaph

阳离子端基肽类脂基因载体的合成

阳离子脂质体作为一种重要的非病毒基因载体,具有一定细胞毒性的缺点,在基因转运方面具有广阔的应用前景.选用脂质体DOTAP和Lipofectamine 2000为基因载体,考察其粒径、Zeta

会议

阳离子脂质体脂质体/DNA复合物基因转运细胞毒性

A G-quadruplex converts wild-type SOD1 into fibrillar aggregates under acidic conditions

Our previous studies suggest that different forms of DNA could act as an accelerating template in the wild-type Cu/Zn superoxide dismutase (wtSOD1) aggregation.

会议

SOD1 aggregationnucleic acidsG-quadruplexfibrilcytotoxicity

Synthesis and DNA-cleaving activities of berberine-based metal complexes

During the past decades, considerable interest has been attracted in transition metal complexes that are capable of binding and cleaving DNA under physiological

会议

Metal complexberberinesynthesisDNA cleavage

Probing Spontaneous Base Flipping of A Single-Mismatched Base Pair in Double-Stranded DNA

Many DNA base repairs and modifications, such as DNA methylation/demethylation, deamination, and hydroxylation, are known to occur extrahelically with the targe

会议

DynamicsSpontaneous single base flippingddFCS

基因编码非天然氨基酸扩展荧光蛋白发色团

与本文相关的学术论文