【摘 要】
：
Pseudanoplocephala crawfordi,a common parasite of free-range pigs and wild boar inhabiting in small intestines,can causes weight loss,growth obstructed,abdominal pain,diarrhea and even death(Jiang et
【机 构】
：
College of Veterinary Medicine,Northwest A&F University,Yangling,Shaanxi Province 712100,China
【出 处】
：
中国畜牧兽医学会兽医寄生虫学分会第十三次学术研讨会
论文部分内容阅读
Pseudanoplocephala crawfordi,a common parasite of free-range pigs and wild boar inhabiting in small intestines,can causes weight loss,growth obstructed,abdominal pain,diarrhea and even death(Jiang et al.,1990;Wang,2002).More importantly,P.crawfordi is an important zoonotic parasite,with 21 human cases identified in Liaoning,Henan,and Shaanxi provinces of China successively,posing significant public health problem.Accurate identification of parasites is central for prevention and control of parasitic diseases.
其他文献
Trichinella spiralis,an intracellular parasite nematode,can cause severe foodborne zoonosis that is known as tichinellosis.The life cycle of T.spiralis consist of adult(Ad),muscle larvae(ML)and newbor
Trichinellosis is a an emerging and reemerging infectious disease,caused by Trichinella spp.Cathepsin F belongs to cysteine protease that is a major virulence factor for parasitic helminths,and it may
Nematophagous fungi are considered to have the best potential as biological agents for the control of gastrointestinal nematodes in domestic animals.However,relatively few studies have been conducted
Vaccination as one of prevention strategies against the Trichinella spiralis infection in hosts is an ongoing effort.However,no ideal vaccine candidates have been identified until now.Therefore,identi
目的 克隆和原核表达脑多头蚴dUTPase基因并分析其酶学活性.方法 从羊脑多头蚴原头节提取总RNA,采用RT-PCR技术扩增脑多头蚴dUTPase基因,将其克隆入原核表达载体pET-32a(+)中,构建pET-32a-dUTPase表达载体,转化大肠杆菌BL21(DE3)后诱导表达,用SDS-PAGE和Western-blot检测表达产物,用免疫荧光组化法检测dUTPase基因在多头带绦虫成虫体
目的 克隆、表达脑多头蚴超氧化物歧化酶(Cu/Zn-SOD)基因并分析其酶学活性.方法 从羊脑多头蚴原头节提取总RNA,采用RT-PCR技术扩增脑多头蚴超氧化物歧化酶(Cu/Zn-SOD)基因,将其克隆入原核表达载体pET-32a(+)中,构建pET-32a-Cu/Zn-SOD表达载体,转化大肠杆菌BL21(DE3)后诱导表达,用SDS-PAGE和Western-blot检测表达产物,并对Cu/Z
青海血蜱为我国的特有蜱种,分布在如青海、宁夏、甘肃、四川、云南和西藏等西部高原地区,是绵羊和山羊特勒虫病的传播媒介,给畜牧业造成严重的经济损失.传统的控蜱方式主要依赖于化学药物,可随着3R(抗性resistance、再增猖獗resurgence、残留residue)问题的出现使得人们不得不寻找化学药物安全有效的替代物.对温血动物无害、环境友好、不易产生耐药性且对蜱具高效杀伤作用的绿僵菌也会成为人们
Spirometra erinaceieuropaei is an important diphyllobothriid cestode whose larval plerocercoid(sparganum)can parasitize human causing sparganosis.In China,sparganosis is an important food-borne parasi
目的 克隆、表达核糖体磷蛋白P2基因并建立诊断山羊脑多头蚴病的间接ELISA诊断方法.方法 提取山羊脑多头蚴总RNA,RT-PCR技术扩增其核糖体磷蛋白P2(TmP2)基因,将TmP2基因克隆入原核表达载体pET-32a(+)中,构建pET-32a-TmP2原核表达载体,转化大肠杆菌BL21(DE3)后诱导表达,用SDS-PAGE和Western-blot检测表达产物,用免疫荧光组化法检测TmP2
半胱氨酸蛋白酶抑制剂家族由一类广泛存在于生物体的半胱氨酸蛋白酶抑制剂组成,分为stefins,cystatins and kininogens三个亚家族。研究报道线虫半胱氨酸蛋白酶抑制剂在寄生虫逃避宿主免疫应答、适应寄生生活中发挥着重要作用。本研究旨在鉴定绦虫的半胱氨酸蛋白酶抑制剂基因家族。首先,利用生物学软件在基因组数据库寻找公布的各种绦虫半胱氨酸蛋白酶抑制剂基因并对其进行结构分析；其次对猪带绦