【摘 要】
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Objective: This study was to explore the anti-fibrotic effect and the possible mechanism of total polysaccharides of Yupingfeng (YPF-P) on bleomycin-induced pulmonary fibrosis in rats.Methods: Pulmona
【机 构】
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Anhui University of Chinese Medicine, Hefei, Anhui, 230038;The First Affiliated Hospital of Anhui Me
【出 处】
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第四届中国药理学会补益药药理专业委员会学术研讨会
论文部分内容阅读
Objective: This study was to explore the anti-fibrotic effect and the possible mechanism of total polysaccharides of Yupingfeng (YPF-P) on bleomycin-induced pulmonary fibrosis in rats.Methods: Pulmonary fibrosis was induced in SD rats by bleomycin (5 mg/Kg), killed 14 and 28 days after BLM administration by abdominal aorta exsanguination and removed the lungs.Lung coefficient was counted at the same time.Besides, H & E and Massons trichrome staining for histopathological changes of lung tissues were observed.Additionally, western blotting and immunohistochemical staining techniques were used to detect expression of TGF-β1, Col-I and α-SMA.Finally, the levels of Col-1 and HYP in lung tissues were also utilized.
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