Synthetic biology is a new area of goal-oriented projects that combine science and engineering.Synthetic biology has two different directions: living entity-based (in vivo) and in vitro.Synthetic pathway biotransformation (SyPaB) is implementation of complicated biochemical reactions by in vitro assembly of numerous enzymes and coenzymes i-3.SyPaB features numerous advantages, such as high product yield, fast reaction rates, easy process control, great engineering flexibility, high product titer, and so on.