Butanol is regarded as important chemical material and liquid fuel, bioconversion of biomass into butanol can feasibly replace chemical transformation.Fermenting cheap lignocellulose to produce butanol is supposed to be more competitive and applicable due to the lack of oil.This paper researched key technical factors in pretreatment, detoxification, enzymolysis and batch fermentation using corn stalks as initial materials.The results were important in bioconversion of non-grain materials.