Objective Cultured of primary vascular endothelial cells from human umbilical veins (HUVECs) and simulated of substrate stiffness about HUVECs.To observe inoculated of primary HUVECs on hydrogel(Gel) by dengue virus-Ⅱ(DV-Ⅱ) infected primary HUVECs induced of IL-29.Methods To isolation and culture of HUVECs, then take experiment by using the well-cultivated fifth generation of HUVECs.Our laboratory had successfully established with Gel of 4Kpa and cells were inoculated on Gel,and infected the primary HUVECs by using the DV-Ⅱ whose MOI=10, then we detected the death rate and infection rate of the cells after 48 hours by using flow cytometry method, along with that, we also inspected the mRNA gene chip, at last, we tested the gene expression result through real-time fluorescent quantitative PCR and double antibody sandwich ELISA detected different of gene expression.Results Detection of cell apoptosis and infection rate by flow cytometry without statistically significant difference(P> 0.05), gene chip and real-time quantitative PCR detected and validated different of IL-29 induced by DV-Ⅱ infected primary HUVEC on Gel.Conclusion We found the appearance of IL-29 and conspicuous discriminating change of it through inoculating the Gel,this might be affected by the interferon antiviral mechanism.