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The genetic variations in internal transcribed spacers (ITS) spanning ITS: 1,5.8S and ITS:2 rDNA of Dicrocoelium dendriticum,isolated from sheep and goats in four geographical regions in Shaanxi province,we -re examined.The lengths of ITS: 1,5.8S and ITS:2 rDNA sequences for D.dendriticum were 749 bp,161 bp,and 234 bp,respectively.Intra:specific sequence variations ofD.dendriticum were 0:0.5% for ITS: 1,and 0:1.3% for ITS:2 rDNA,while the inter:specific variations among species in genus Dicrocoelium in ITS:2 rDNA were 3.4: 12.3%.Phylogenetic analysis based on sequences of ITS:2 rDNA showed that all D.dendriticum isolates in the present study were grouped with reference D.dendriticum isolates from sheep and goat,and D.dendriticum iso -lates from cattle and Japanese serow were clustered in a sister clade.However,the phylogenetic tree could not reveal geographically genetic relationships of D.dendriticum isolates in different origins and hosts.These find -ings provided basic information for further study of molecular epidemiology and control of D.dendriticum inf -ection in Shaanxi province as well as in the world.