Although 316L stainless steel(316L SS) has been widely used as biomedical materials, the hemocompatibility is also a challenge while it used as blood contact biomedical materials. Surface modification of 316L SS can maintain the bulk properties of metal but optimize the surface properties to improve the hemocompatibility. In this paper, Titania (TiO2) and carbon nanotube (CNT) doped TiO2 thin films were deposited on glass and 316L SS substrates by sol-gel dip coating method. The microstructures of the films coated on 316L SS were characterized by X-ray diffraction (XRD) and scanning electron microscopy (SEM) with energy dispersion X-ray analysis (EDX). The films are crystalized anatase phase with smooth surface. The band gap of undoped and CNT-doped TiO2 films is about 3.4 eV. The electrochemical evaluation is conducted in simulated body fluid by potentiodyn mic polarization experiment and it shows the CNT-doped TiO2 film coated 316L SS has the best corrosion resistance. Static contact angles are carried out by different liquid. The coatings are hydrophilic in nature. The surface energies and interface tension of the materials with the blood compositions are calculated and the CNT-doped TiO2 film had lower interface energy with the blood compositions, which indicated good hemocompobilitily. CNT-doped TiO2 films has potential application for 316L SS in blood contact biomedical devices.