干细胞核心基因Nanog在乳腺癌干细胞及非干细胞中的表达与定位

来源 :第十八届全国临床肿瘤学大会暨2015年CSCO学术年会 | 被引量 : 0次 | 上传用户:kaless
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  目的:研究干细胞核心基因Nanog在乳腺癌干细胞(BCSCs)和非乳腺癌干细胞(Non-BCSCs)中的表达及定位差异以及BCSCs在MCF-7微球体中的分布情况。方法:应用Nanog免疫荧光、免疫细胞化学及与Hoechst33342荧光染料双染色方法检测BCSCs和Non-BCSCs中Nanog的表达及其定位。
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