Rhodococcusaetherivorans ZJB 1208腈水合酶的克隆与表达

来源 :2015年上海市研究生学术论坛(生物化工) | 被引量 : 0次 | 上传用户:ltc835634161
下载到本地 , 更方便阅读
声明 : 本文档内容版权归属内容提供方 , 如果您对本文有版权争议 , 可与客服联系进行内容授权或下架
论文部分内容阅读
  腈水合酶(Nitrile hydratase,EC4.2.1.84)能够催化腈化合物水合生成相应的酰胺,具有反应条件温和,催化效率高以及区域、立体选择性严格等优点,是一类重要的生物催化剂.腈水合酶酶法生产丙烯酰胺开创了生物催化在大宗化学品合成中应用先河.尽管腈水合酶已被广泛应用,但其异源高效表达难度极大,限制了该酶的商品化生产.由于腈水合酶基因在野生菌株中以基因簇的形成存在,由β亚基与α亚基等构成多聚体金属酶等特点.
其他文献
The newbom immune system is characterized by an impaired Thl-associated immune response.Microbial colonization after birth and vaccination with live vaccines ha
会议
会议
会议
Mesenchymal stem cells (MSCs), also known as multipotent mesenchymal stromal cells, exist in most tissues and are a key cell source for tissue repair and regene
会议
Innate immunity evolved to recognize microbial infection and to respond to danger signals that appear under disease conditions.The most recently described innat
会议
会议
Our body equips a number of cytoprotective systems that sense environmental insults and activates cellular-defense-enzyme genes.Transcription factor Nrf2 is ess
会议
Antigen-presenting cells (APCs) including dendritic cells (DCs) play critical roles in the initiation of immune response and maintenance of immune tolerance.Rec
会议
会议
会议