Oryza sativa and Brassica napus-two important crops for food and oil, respectively-share high seed yield as a common breeding goal.As a model plant, O.sativa genomics have been intensively investigated and its agronomic traits have been advanced.In the present study, we used the available information on O.sativa to conduct comparative mapping between O.sativa and B.napus, with the aim of advancing research on seed-yield and yield-related traits in B.napus.Firstly, functional markers (from 55 differentially expressed genes between a hybrid and its parents) were used to detect B.napus genes that co-localized with yield-related traits in an F2∶3 population.Referring to publicly available sequences of 55 B.napus genes, 53 homologous O.sativa genes were subsequently detected by screening, and their chromosomal locations were determined using silico mapping.Comparative location of yield-related QTL between the two species showed that a total of 37 O.sativa and B.napus homologues were located in similar yield-related QTL between species.Our results indicate that homologous genes between O.sativa and B.napus may have consistent function and control similar traits, which may be helpful for agronomic gene characterization in B.napus based on what is known in O.sativa.