Three UV A/B resistant diesel oil-enriched microbial communities,UV1,UV12 and UV24,were established by adding 1%diesel oil to the sea water/sea sand collected from the diesel oil contaminated coastline for enrichment after UV A/B radiation for 1,12,and 24 hours,respectively.Molecular methods(DGGE and cloning)and traditional isolation method were used to investigate bacterial and yeast members in these communities.DGGE patterns indicated that UV12 and UV24 communities were different from that of the UV1 community.Gene sequencing of DGGE bands indicated that the main bacteria and yeasts in the UV12 and UV24 communities were Dietzia sp.and Sarocladiumsp.,respectively.A total of 56 bacterial OTUs and 31 fungal OTUs were obtained from the clone libraries of these three communities.Bacteria in the phylum of α-Proteobacteria,β-Proteobacteria,γ-Proteobacteria,Bacilli and Actinobacteria,yeasts in the phylum of Agaricomycetes,Dothideomycetes,Eurotiomycetes,Sordariomycetes and Saccharomycetes were detected in the clone libraries constructed from these three UV resistant communities.The most dominant bacteria were Alcaligenes sp.,Ochrobactrum sp.,and Dietzia sp.,while the most dominant yeasts were Granulobasidium sp.and Sarocladium sp.in the UV 12 and UV24 communities.66 bacterial strains and 21 yeasts strains were isolated from the UV24 community.All the isolated strains,especially the yeasts showed high UV resistance.Among the isolated bacterial strains and yeast strains,Ochrobactrum anthropi FCH 18 and Candida tropicalis SA-4,respectively had the highest diesel oil degrading ability.