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采用无菌溶液培养试验,研究了两个小白菜品种(‘金申’与‘五月慢’)对硝态氮(NO3--N)、甘氨酸态氮(Gly-N)吸收的动力学规律,以及NO3--N对Gly-N吸收动力学参数的影响。结果表明,两个小白菜品种对不同氮源的吸收都符合米氏方程,具有主动吸收的特征,Vmax及1/Km值均为NO3-N>Gly-N,Gly-N吸收的Vmax及1/Km值‘金申’大于‘五月慢’。NO3--N的存在抑制了小白菜Gly-N的吸收速率、Vmax及1/Km值,其中品种‘五月慢’Gly-N吸收的Vmax下降显著,品种‘金申’下降不显著。常规水培10 d后,小白菜干重、氮积累量对NO3--N与Gly-N处理表现显著差异,均为NO3--N>Gly-N。品种间差异显著,Gly-N处理下‘金申’干重及氮积累量显著大于‘五月慢’。综上,小白菜对于有机、无机态氮的吸收能力存在基因型差异,‘金申’品种较‘五月慢’品种更能适应有机氮源的培养环境。
The kinetics of NO3 - N and Gly-N uptake by two Chinese cabbage cultivars (’Jinshen’ and ’May slow’) were studied using sterile solution culture experiments , And NO3 - N on Gly-N uptake kinetic parameters. The results showed that the absorption of different nitrogen sources in two Chinese cabbage cultivars accorded with Mie equation and had the characteristics of active absorption. The values of Vmax and 1 / Km were NO3-N> Gly-N, Vmax of Gly-N and 1 / Km value ’Jin Shen’ is greater than ’slow in May’. NO3 - N inhibited the absorption rate, Vmax and 1 / Km of Gly-N, and the Vmax of Gly-N uptake in May slowed down significantly. The decline of Jin Shen was not significant. After conventional hydroponic culture for 10 days, the NO3 - N and Gly - N contents of dry matter and nitrogen accumulation of Chinese cabbage were significantly different, both of which were NO3 - N> Gly - N. There was significant difference among cultivars. The dry weight and nitrogen accumulation of ’Jin Shen’ under Gly-N treatment were significantly greater than those of ’May slow’. In summary, there are genotype differences in the absorption capacity of Chinese cabbage for organic and inorganic N, which is more adaptable to the cultivation of organic N sources than that of May.