The cell sheets detached from thermo-sensitive hydrogel surface have potential applications in biomedical fields, such as organs or tissue regeneration and cancer or disease therapy The silk sericin/poly(N-isopropylacrylamide) nanocomposite hydrogel with semi-interpenetrating network structure were prepared by in-situ free radical polymerization in aqueous solution by using nanoclay minerals lithium magnesium silicate hydrate(LMSH) as physical cross-linker.