Preparation and in vitro evaluation of aptamer conjugated lipid-polymer hybrid nanoparticles for MUC

来源 :2014年中国药物制剂大会——中国药学会药剂专业委员会年会、第六届亚洲阿登制药技术研讨会暨国际控释协会中国分会年会 | 被引量 : 0次 | 上传用户：myloft1d

【摘要】

：

【作者】

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Zhenbao Liu Huanzhe Zhao Juewen Liu Jinsong Ding

【机构】

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School of Pharmaceutical Sciences,Central South University,Changsha 410013,Hunan Province,People's

【出处】

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2014年中国药物制剂大会——中国药学会药剂专业委员会年会、第六届亚洲阿登制药技术研讨会暨国际控释协会中国分会年会

【发表日期】

：

2014年8期

【关键词】

：

lipid-polymer hybrid nanoparticle aptmer tumor targeting and therapy

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　　Objective: In this research, a novel vinorelbine-loaded aptamer (Apt) conjugated lipid-polymer hybrid nanoparticles (Apt-NP/VRL) were constructed for specific targeting MUC 1 protein over expressed tumor cells.Methods: Apt-NP/VRL were prepared by self-assembling method using lecithin, DSPE-PEG2ooo-COOH, and poly (lactic-co-glycolic acid) (PLGA) as carrier material.The formulation was optimized by orthogonal design.The aptamer was conjugated to DSPE-PEG2000-COOH through EDC and NHS coupling (Figure 1).Though changing the ratio of DSPE-PEG200o-Apt, NPs with different aptamer densities were synthesized and characterized.Courarin-6 labeled nanoparticles (Apt-NP/Cou-6) were prepared to evaluate the cellular uptake efficiency.The anti-tumor activity of Apt-NP/VRL to MCF-7 cell and HpG2 cell was evaluated.

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