【摘 要】
：
目的:探索幽门螺杆菌临床标本的最佳运送条件和最适培养基.方法:将实验菌株加入小牛血清、牛奶运送液,分别置不同温度及气体环境6h后接种于哥伦比亚血琼脂微需氧培养;将20例临床标本分别接种于MH和哥伦比亚血琼脂微需氧培养5天,比较分离效果.结果:血清中活菌数多于牛奶中活菌数,微需氧和烛缸运送下活菌数多于空气和有石蜡油覆盖的运送液中活菌数,微需氧37℃(或26℃)的血清中活菌数多于6℃下的活菌数(P＜0
【机 构】
：
贵州医科大学基础医学院微生物学教研室,贵阳 550025 贵州医科大学附属医院消化内科
论文部分内容阅读
目的:探索幽门螺杆菌临床标本的最佳运送条件和最适培养基.方法:将实验菌株加入小牛血清、牛奶运送液,分别置不同温度及气体环境6h后接种于哥伦比亚血琼脂微需氧培养;将20例临床标本分别接种于MH和哥伦比亚血琼脂微需氧培养5天,比较分离效果.结果:血清中活菌数多于牛奶中活菌数,微需氧和烛缸运送下活菌数多于空气和有石蜡油覆盖的运送液中活菌数,微需氧37℃(或26℃)的血清中活菌数多于6℃下的活菌数(P＜0.05);置微需氧和烛缸的运送液中活菌数无明显差异(P＞0.05).20例标本经MH血琼脂培养阳性率(75％)高于哥伦比亚血琼脂阳性率(45％)(x2=4.401,P＜0.05).结论:胃黏膜标本可置于小牛血清运送液于微需氧条件(或烛缸)下运至实验室分离培养;初次分离以10％绵羊血MH琼脂为佳.
其他文献
植物乳杆菌(Lactobacillus plantarum)是发酵食品中一种常见的益生菌,群体感应(quorum sensing,QS)是细菌进行信号交流的一种普遍方式,Lb.plantarum是否通过QS介导其益生特性尚不清楚.本研究首先运用实时相对半定量PCR方法,研究了YM4-3在不同生长阶段luxS基因(合成QS信号分子AI-2的关键基因)的表达情况;然后进行了YM4-3在指数早期、指数中
目的 研究澳门红树林植物内生放线菌多样性,以期发现新型药用微生物资源,为发现新型抗生素奠定基础.方法 从澳门路氹城生态保护区采集12份红树植物样品;植物样品经表面消毒粉碎后,采用10种分离培养基,进行铺板、培养和分离纯化;对纯化到的菌株进行16S rRNA基因的PCR扩增、测序,序列比对.结果 共分离到193株放线菌,分布在放线菌纲的9个目18个科33个属,除链霉菌属为优势菌属外,还发现了Amni
Bio-control agent such as Iturin A is natural antifungal agent wildly used in agriculture.In the present study,a new modified chitosan submicron,succinaldehydic acid (SAC)-chitosan (CS) was synthesize
Fusarium wilt disease in cucumber is widespread and is responsible for serious economic losses.An antagonistic actinomycete strain CT205,isolated from the soil of Zijin mountain in Nanjing,China,stron
Peanut shells as a kind of tested adsorption material,the influences of key factors (pH,reaction duration,initial concentration and adsorption dosage) on its adsorption of heavy metal ions including P
Optically active diaryl alcohols is an important class of chemical chiral compounds which could be applied in the synthesis of natural products,pharmaceuticals and agrochemicals,such as antihistamine,
HNH proteins are a very common small nucleic acid binding proteins generally associated with endonuclease activity and are found in all kingdoms of life.Although HNH proteins from mesophiles have been
Altemariol (AME),temariolmethylether (AOH),and altenuene (ALT) were the common mycotoxins product by Alternaria alternatain.Those mycotoxins also cause the plant disease and threat to people health.A
Clostridium acetobutylicum is a native producer of ABE (acetone,butanol and ethanol) solvents,among which 1-butanol is an important bulk chemical and also one of the most promising biofuels.The metabo
As an excellent exoelectrogen in Microbial Fuel Cell,the genomes of Shewanella were significant in exoelectrogen genomics research.In this paper,we performed a comprehensive re-annotation of protein-c