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The presence of marker genes, especially antibiotic resistance, in GM plants is of concern in society due to fears associated with risks for the environment and human health.Creation of transgenic plants that do not contain the foreign genetic material, especially bacterial and viral origin largely alleviates the tension.Here we used the pMF system (Plant Research International, Wageningen, Netherlands) containing of the Z.rouxiirecombinaseR and a CodA-nptIIbi-functional selectable gene for produce marker-free transgenic apple plants carrying the super sweet thaumatinII gene from tropical plant Thaumatococcusdaniellii under control of E8 gene fruit specific promoter and rbsS3A terminator.