Coupling of chemiluminescence detection with micro-scale separation technologies

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  Capillary electrophoresis (CE) and microchip capillary electrophoresis (MCE) have become very important micro-analytical methods in many fields such as biology, biomedicine, and environmental and food sciences.However, the small injection volume makes it a challenge to achieve high detection sensitivity.Chemiluminescence (CL) is a highly sensitive detection method widely used in flow injection analysis, high performance liquid chromatography and immunoassay.Due to its simple optical system and low background nature, this method is uniquely suited to on-line detection for CE and MCE.CE and MCE coupling with CL detection system exhibit certain advantages from the combination of CE with a highly separation efficiency and CL with a highly sensitivity.Recently, we applied the CE-CL and MCE systems successfully in separation of metal ions, amino acids and proteins, in immunoassay and in single cell analysis.
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