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Picrorhiza scrophulariiflora Pennell (P.scrophulariiflora) is widely used in the traditional Chinese medicine for the treatment of damp-heat dysentery, jaundice and steaming of bone.In this paper, a potential ionic liquid-based ultrasonic-assisted extraction (ILUAE) method was developed for efficient sample preparation and a characteristic ion screening strategy with ultra-performance liquid chromatography coupled to electrospray ionization quadrupole time-of-flight mass spectrometry (UPLC/ESI-QTOF-MSn) was established for rapid identification of glycosides in P.scrophulariifora.