【摘 要】
：
Two N2-fixing bacteria isolates,B11S and B8S,were obtained from the surface-sterilized stalks of sugarcane cultivars GT11 and B8.Both isolates showed acetylene reduction activity in nitrogen-free medi
【机 构】
：
State Key Laboratory for Subtropical Agri-Bioresources Conservation and Utilization,College of Agric
【出 处】
：
广西甘蔗学会2016年年会暨学术交流会
论文部分内容阅读
Two N2-fixing bacteria isolates,B11S and B8S,were obtained from the surface-sterilized stalks of sugarcane cultivars GT11 and B8.Both isolates showed acetylene reduction activity in nitrogen-free media.The two nitrogen-fixing bacterial isolates produced siderophores,and the ability of B8Ss was higher than that of B11S.The two isolates were also able to dissolve Ca3(PO4)2,and the ability of B11S was higher.It was found that only the strain B8S displayed ACC deaminase activity.The IAA production ability of strain B8S was higher than that of B11S.Both the B11S and B8S isolates were classified on the basis of 16 S rRNA gene sequences and their biological characteristics.The 16 S rRNA gene sequence of isolates B11S and B8S showed 98.5 and 100%similarities with Stenotrophomonas maltophilia and Agrobacterium tumefaciens(Rhizobium radiobacter),respectively.For both growth and nitrogenase activity of the isolates,the optimum temperature was 31℃.The optimum pH for their growth was 6.0,but it ranged from 6.5 to 7.0 for nitrogenase activity.The two strains grew faster and showed increased nitrogenase activity with increasing concentration of carbon in the cultural media,for which the sucrose was better compared to glucose.Further,the growth and nitrogenase activity of the strains were found better when cultured in the media containing suitable concentrations of NH4+and NO3-; however,the growth of both isolates was hindered significantly with increasing concentration of both nitrogen sources.
其他文献
In order to investigate polysaccharide accumulation in sugarcane leaves under azotobacter inoculation and nitrogen supply,a pot culture experiment was conducted in 2012.The plants of sugarcane varieti
Sugarcane(Saccharum officinarum L.)is an important crop for sugar and bioenergy worldwide.The increasing greenhouse gas emission and global warming during climate change result in the increased freque
Ascorbate peroxidase(APX)is a key enzyme for active oxygen scavenging of higher plants,and it plays an important role in plants against abiotic stresses.In this study,a thylakoidal APX(TAPX)gene was c
NifK gene is a key nitrogenase and plays an important role in the nitrogen fixation process.It is necessary to obtain the nifK gene for exploring its relationship with nitrogen fixation.This study aim
Water stress causes considerable yield losses in sugarcane.To investigate differentially expressed genes under water stress,a pot experiment was performed with the sugarcane variety GT21 at three wate
Stem borer incidences in sugarcane result in huge economic losses to sugarcane cultivation worldwide.Genetically modified(GM)sugarcane with stem borer resistance has good prospects for commercializati
Browning to death is a major limitation for sugarcane tissue culture.This study was mainly carried out to investigate the influence factors which relate to phenolic browning and analyze the relationsh
Soil survey was conducted at four different locations of Guangxi,China to investigate the effect of peanut and soybean inter-cropping in sugarcane cultivation on the soil properties and diazotrophic b
Sugarcane variety ROC22 was used as the experimental material and water stress treatment was performed for the 4 or 5-leafed plants with 25%polyethylene glycol(PEG)6000 solutions.The cDNA sequence of
Sucrose synthase is one of the key enzymes involved in sucrose metabolism.In order to increase the sucrose content at the molecular level,it is very necessary to understand the biological function of