Eleven Bacillus thuringiensis isolates were recovered from phylloplanes of Magnolia denudata,a specific source of new strains of B.thuringiensis.Among these,a new strain,LLP29,was found to be most toxic to mosquitoes based on the results of preliminary toxicity analysis.Phase contrast microscopy,mosquitocidal activity and PCR analysis were performed to learn more about the characteristics of this novel mosquitocidal isolate.