Aim Some indirect evidences indicate a possible correlation between oxidative stress and motion sickness.The aim of this research was to investigate whether oxidative stress contributing to motion sickness in mice or not.Methods We examined the mRNA levels of peroxiredoxin 6 (PRDX6), catalase and enzyme superoxide dismutase 1 (SOD1) ; reactive oxygen species (ROS) ; total antioxidant capacity and SOD activity in different brain regions after rotary stimulation.Mice motion sickness index was recorded after rotation when pretreated with paraquat, vitamin C or vitamin E.Results The ROS level and antioxidant capacity were both increased in cerebellum plus brainstem (CB) after rotation, a critical region determines motion sickness.However, manipulation of oxidants or antioxidants using pharmacological method in vivo had no influence on motion sickness index in mice.Conclusion Oxidative stress is not involved in the development of motion sickness in mice.