【摘 要】
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Objectives To study the effects and molecular mechanisms of advanced glycation end products (AGEs) on arteriosclerosis (AS) after kidney transplantation.Methods We collected serum samples from 30 heal
【机 构】
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Department of Urology,The First Affiliated Hospital of Nanjing Medical University,210029
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Objectives To study the effects and molecular mechanisms of advanced glycation end products (AGEs) on arteriosclerosis (AS) after kidney transplantation.Methods We collected serum samples from 30 healthy volunteers and 30 renal transplant recipients and tested for levels of AGEs and serum creatinine.We also collected arterial specimens and observed histological changes, calcium deposition, α-smooth muscle actin (α-SMA) and osteopontin (OPN) expression.Moreover, We used western blotting to analyze protein levels of α-SMA, OPN and Integrin-linked kinase (ILK) in rat vascular smooth muscle cells(VSMCs) after treatment with AGEs.Then, we observed ILK, α-SMA and OPN expression in ILK plasmid transfected rat VSMCs and with AGEs stimulation or not.Finally, we detected expression of ILK and receptor for advanced glycation end products (RAGE) in rat VSMCs dealed with AGEs and anti-RAGE antibody or not.
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