In addition to providing energy,carbohydrates also participate other biological activities and functions [1] and have become an important subject of research,which is comparable to DNA and proteins.However,carbohydrates have neither ultraviolet absorption groups nor fluorescent structure,their analysis with normal chromatographic methods is still a challenge.Here in microchip electrophoresis was used for the rapid and efficient separation and determination of monosaccharide and oligosaccharides.Low-cost cyclic olefin copolymer microchips were adopted to reduce the cost of the analysis and 8-aminonaphthalene-1,3,6-trisulfonate (ANTS) was used as fluorescent labeling reagent to obtain high sensitivity with laser induce fluorescence detection xylose,glucose and lactose were used as model compounds for the optimization of separation conditions.The results showed that hydroxypropyl cellulose (HPC) in the borax buffer could provide excellent resolution of these saccharides within 20 s.The detection limits was 0.15-0.23 μM and the linear relationship was found in the range of 0.5-500 μM.The number of theoretical plates number could be 1.0×106 /m.Relative standard deviations of peak areas and migration times were ≤ 3.12% and 1.94% respectively.The method was tested by the determination of glucose in human urine and blood (Figure 1).Reasonable standard addition recoveries of 80.1%-108% were obtained.The method was also used to determine the oligosaccharides in Konjac (Figure 2),a component which is believed to have functions of blood glucose reduction,and treat cardiovascular disease prevention,free radical scavenging [2].These results proved that low-cost microchip electrophoresis could be a cheap and rapid alternative for saccharides analysis.