目的：To investigate the regulation of endothelial cell(EC)microRNAs(miRNAs)altered by heat stress,miRNA microarrays and bioinformatics methods were used to determine changes in miRNA profiles and the pathophysiological features of differentially expressed miRNAs.方 法：Thirty-one differentially expressed miRNAs were identified,including 20 down-regulated and 11 up-regulated miRNAs.结果：Gene Ontology(GO)enrichment analysis revealed that the validated targets of the differently expressed miRNAs were significantly enriched in gene transcription regulation.The pathways were also significantly enriched in Kyoto Encyclopedia of Genes and Genomes(KEGG)analysis,and most were cancer related,including the MAPK signaling pathway,pathways involved in cancer,the Wnt signaling pathway,the Hippo signaling pathway,and proteoglycans involved in cancer and axon guidance.The miRNA-gene and miRNA-GO network analyses revealed many hub miRNAs,genes and functions.Notably,miR-3613-3p exhibited a dominant role in both networks.Mitogen-activated protein kinase kinase kinase 2(MAP3K2),alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase A(MGAT4A),transforming growth factor beta receptor 1(TGFBR1),ubiquitin-conjugating enzyme E2 R2(UBE2R2),and Sma-and Mad-related family 4(SMAD4)were most likely to be controlled by the altered miRNAs in the miRNA-gene network.The miRNA-GO network analysis revealed significantly complicated relationships between the miRNAs and functions and that the significantly enriched functions targeted by the differently expressed miRNAs were mostly involved in regulating gene transcription.结论：Our study demonstrates that miRNAs are involved in the pathophysiology of heat-treated ECs.Understanding the functions of miRNAs provides novel insights into the molecular mechanisms underlying the heat-induced pathophysiology of ECs.